Gel Electrophoresis

Gel Electrophoresis Procedure We began the experiment by seal the ends of the gel- molding tray with tape, and inserting the comb. We placed the tray out of the way to make sure that it was non disturbed. We poured about 6mm of agarose solution into the casting tray. The gel covered alone about 1/2 the height of the combs teeth. We re force outd self-aggrandising bubbles with the tip of a transfer pipette while the gel was still a liquid. While waiting about hug drug minutes for the gel to solidify, we made sure not to move the casting tray.

After the gel solidified we unsealed the ends of the casting tray. We placed the tray in the gel box, so that the comb was at the negative end. We poured 250ml of tris-borate-EDTA (TBE) buffer into the gel box to a take aim that covered the entire surface of the gel. We gently removed the comb, qualification sure not to rip the wells. The sample wells left by the comb were completely submerged. We care profusey used the pipet to load the DNA into th...If you want to get a full essay, order it on our website: Ordercustompaper.com

If you want to get a full essay, wisit our page: write my paper